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摘 要：[目的]采用稀釋涂布分離法研究了不同熱預(yù)處理時(shí)間對放線菌分離效果的影響。[方法]在120℃加熱預(yù)處理下，分別設(shè)置了30min、45min、60min、75min、90min、105min幾種不同熱預(yù)處理時(shí)間試驗(yàn)對放線菌分離的影響。[結(jié)果]隨著預(yù)處理時(shí)間的延長，放線菌的數(shù)量和種類均呈先升高、后降低的趨勢，而雜菌的數(shù)量呈降低的趨勢。120℃加熱預(yù)處理75min，放線菌分離效果最好。[結(jié)論]適當(dāng)?shù)臒犷A(yù)處理有利于放線菌孢子萌發(fā)，相對提高放線菌的出菌率，同時(shí)對雜菌生長有一定抑制作用。 關(guān)鍵詞：放線菌；分離效果；熱預(yù)處理
Abstract: [Objective]The influence of different time of thermal pretreatment to the isolation efficiency of actinomycetes was studied by plate paint isolation methods.[Methods]At 120℃ thermal pretreatment,30mi-n、45 min、60min、75 min、90 min、105 min were setted to test the influence of different time of thermal pretreatment on the isolation of actinomycetes.[Results]With the extension of time, the quantity and types of actinomycetes first increased and then decreased, but the quantity of bacteria was relatively reduced. With thermal pretreatment 120℃,75min,we got the best effect on selective isolation of actinomycetes.[Co-nclusion]Appropriate thermal pretreatment might promote actinomycetes spore germination and improve t- he isolation results relatively and had a inhibition on bacteria. Key words:actinomycetes; isolation efficiency; thermal pretreatment |
