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| 折扣與優(yōu)惠：團(tuán)購最低可5折優(yōu)惠 - 了解詳情 | 論文格式：Word格式(*.doc) |  |
摘 要:目的：本實驗主要研究一種能快速提取、凈化茯苓中異丙威并檢測其含量的方法。 方法：異丙威是一種氨基甲酸酯類農(nóng)藥。本實驗采用高效液相色譜法對異丙威的含量進(jìn)行測定，選取超聲波輔助提取茯苓粉中的異丙威，考察了無水硫酸鈉和弗羅里硅土不同比例作固相萃取柱對其凈化的影響，不斷改變流動相比例以及柱溫，比較其對測定的影響，確定了高效液相的色譜條件。 結(jié)果：色譜柱（Ultimate XB-C18 5μm 4.6×250mm)；柱溫：30℃；紫外檢測器波長：210nm；進(jìn)樣量：10μl；流動相：乙腈：一級水=55：45（V/V）；流速：1.00ml/min；經(jīng)計算，該方法的回收率為：96.87％，相對標(biāo)準(zhǔn)偏差為：11.65％，實驗證明該方法效果好。 關(guān)鍵詞：異丙威；高效液相色譜法；茯苓
ABSTRACT:Objective：This paper want to find a fast and accurate method to detect isoprocab from Poria cocos。 Methods：Isoprocarb is a carbamate pesticide。The ultrasonic wave was employed to extract isoprocarb from Poria cocos，the purify condition also been investigated，the contents of isoprocarb was been detected by HPLC。The proportion of Na2SO4 and Florida，which as the solid-phase，was investigated the effect of the compounds which extracted from Poria cocos，the result shown the purification column included 0.4g Na2SO4，1g Florida，0.4g Na2SO4。 The detection method were investigated the column temperature，mobile phase and its proportion，detectin wavelength and velocity for HPLC。The recovery and the loss rate also studied in this paper。 Results：Chromatographic column (Ultimate XB-C18 5μm 4.6×250mm)； Column temperature：30℃；Ultraviolet detector wavelength：210nm；Enter the sample weight：10μl；Mobile phase：acetonitrile：level 1 water = 55：45 (V/V)；Velocity：1.00 ml/min；The recovery is 96.87％，RSD is 11.65％。This method worked well。 Keywords： Isoprocarb；HPLC；Poria cocos |
